Journal of Nanjing Railway Medical College 2000; 1928385 verapamil to non-Gundam cell lines Chen Baoan, Cheng Jian, Gao Feng, Dai Meimei, Jin Baocui, Jiahua, Gao Chong, Jiang Zao Department of Hematology and Oncology, Affiliated Hospital of Nanjing Railway Medical College. Nanjing, Jiangsu 2100091, Abstract purpose, to detect the beaten Dingqing. For non-plants, the sensitization and drug reversal of this strain of Gundam fine are used. Methods The methicone yellow colorimetric method was used to test the drug sensitivity. It is now observed that the sensitization and drug resistance of Etoposide 4, Shan, 16 were reversed. The result is turbid 1. Right.
6, Huang Jie, Shi Xi Da strains are, 31 and 39., called. Single 0516 pairs of muscle 6, fine hair 5, for 247.17.1. Add 5 post ten, deal with forget 6 pairs, cell, the value drops. The original 73 sheep muddy, 6 pairs, 60. do fine hair 10 Ling 872.97; 4. Add 51 to use. After 7-1 pretreatment, the sink decreased to 208.45.1, which was the original 14.191 and the nuclear close to 160 sensitive strains of 16 pairs. in conclusion. 1 against Africa, up to 1 fine, with sensitization and drug resistance reversal.
1.3 Reagents, 71 kits are German Bollingman's product culture medium is the United States 01800 company's product, calf serum is Hangzhou Jiqing literature identification code person article number 10072752002833 company product.
1.4 The drug sensitivity test adopts methoxazole yellow colorimetry, 1 to 24. Inoculate the cells of the plant with a well-proliferated final concentration into the light-well culture plate. There are 3 parallel holes for each drug concentration in 100 holes, and a blank control group 640 culture medium and a normal control group containing melon 60 cells without drugs are placed under 37, volume fraction of 5, 5 and 32 under full humidity. Remove the culture plate after cultivating for 48 hours, add to each well, mark 択 åˆ æ¶² 5, 〃 Thaw the lamp private record liquid and Tingzhuo coupler before use, value, and use the blank control to zero during testing.
When observing the effects of sensitization and reversal, select a constant, pretreatment with 5 tower concentration. Plus + concentration. 16 = 1.5 Statistical analysis adopts paired test, and the 5 values ​​of sinks are calculated by comprehensive calculation method 3.
2 Results The inhibition rate of 160 and melon 60 cells showed a trend of increasing toxicity at a concentration of 51.17; when the concentration of 1 and 4 were 1, the 60. cells showed no obvious linearity, and the trend of increasing toxicity at the 1st and 40th plants. 1.
Fund project The Ministry of Railways Science and Technology Fund funded the project only 7031 touch, Mu, 13, 0 touch, 戳 霨 imperial Tibetan bond, the inhibition rate of 16, and melon 60 six office cells n 2.2 on 16 sensitization effect. With 715 tower, 1 after pretreatment, 6 pay a few 6, cells 1.73 times, 2.
Circumstance 2, enhance the inhibition rate of Ping 16 on muscle 60 cells, 2.3 reverse the drug resistance of 16, medicinal treatment with 5 wow, 1 after pretreatment, under 16 pairs of 60 cells. The value decreased from 827.97 to 208.45, 1. The reversal multiple was 4.19 times, 3.
3 Discussion, 1 as a classic multidrug resistance reversal 1 was reported in 1981. Over the years, people have studied the man's broom for its action mechanism, and believe that if it is through competition to suppress sugar for white power, it is the earliest discovered multi-drug resistance, 1 cold egg, 1 cold egg, and up to cell line. We have established the Yi 71 method to detect the sensitivity of tumor cells, and confirmed that their sensitivity stability is better than the Wei 1 method, and the operation is simple. In this experiment, the Yi method was used to detect drug sensitivity, and it was confirmed that it can be strong, 16 to 160 cells. Sensitivity can also be reversibly used to explain the resistance reversal effect of 1 by competitively binding to 1 glycoprotein, and its specific mechanism of action remains to be studied. We have made a preliminary exploration in this regard, and believe that it can enhance the induction of cell apoptosis of chemotherapeutic drugs, and cell apoptosis-related genes 12 may be the target of its effect. This experiment also confirmed that it can be used as 1 that is up to In-depth research, more and more drug resistance reversal agents have been developed. Like or because of side effects. The reversal effect of Cheng〃1 is not obvious, or due to various reasons such as high price, so far, people have not found an ideal drug resistance reversal agent. This study believes that the mechanism of drug resistance reversal of the 3 glycoprotein outer ice is still left. As the earliest discovered drug resistance reversal agent, it still has broad application prospects. Due to its significant and stable reversal effect, it is a good control for drug resistance reversal experiments. Although serious cardiac side effects limit its clinical application, it has been researched that the main side effects of the heart are its right-handed components, and At present, most of them on the market are mixed, and now there are left-handed abroad, and their cardiac side effects are reduced by 801. This also opens up a new way for the study of resistance reversal of anti-tumor drugs. , Do n’t concentrate in the cell and increase it with everyone. The sensitivity of cytotoxic tumor drugs is selected in this experiment as the cell line called “no s, lul, lt, traznlimlnnna.ma, sugar egg, and sundaâ€. Elephant. Among them 160tiihin 5 Xu Shuru Lian. Chen Xiu Pharmacology Zhang Fanghua 7 2nd Edition. Beijing People's Medical Publishing House. 1992.203.
I6, Baoan Chengjian. high. Wait. Methazole yellow colorimetric method was used to detect the drug sensitivity of tumor rib cells. Chinese Medical Laboratory Journal, 19, 222566.
7 Cheng Jian. Ask Baoan. high. Wait. Different; Introduce a preliminary discussion on the mechanism of promoting 16 induced 160 cell modulation. Leukemia, 1999.52824.
Received date 19929 Study on liver weight and functional recovery after liver resection in cirrhotic rats Li Junsheng, General Surgery, Affiliated Hospital of Nanjing Railway Medical College, Bo Zhenling, Nanjing 219, Jiangsu, Abstract 1 Objective To study partial hepatectomy in cirrhotic rats The relationship between post-hepatic weight recovery and function 5; method to prepare liver cirrhosis with TfSjMfcJ3tULWfLqMj function in this valley, 4-lead and no significant improvement compared with treatment such as Qi. Conclusion Liver cirrhosis is a big deal. Livestock can be regenerated. However, in the short term, sweat function, liver cirrhosis resection, long liver regeneration function; rat 1 middle model number 1575.2 document identification code 4 fund project Ministry of Railways Science and Technology Fund Project Langji 016
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