UV-1100 UV spectrophotometer for the detection of thiophanate-methyl and carbendazim in fruit

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Ultraviolet Spectrophotometric Method for Detecting Thiophanate-Methyl and Carbendazim in Fruits

This article describes the use of a UV spectrophotometer for detecting thiophanate-methyl and carbendazim in fruits. The instruments used include UV-1100 and UV-1200. This method is widely applied in food safety analysis to ensure that fruit products meet quality and regulatory standards.

Width="835" height="338" src="http://i.bosscdn.com/blog/nophoto.gif" /></p> The procedure involves extracting the sample using dichloromethane. After extraction, the solution is neutralized with sodium hydroxide and re-extracted to isolate the target compounds. The absorbance at 282 nm is measured using a 1 cm quartz cuvette, and standard curves are created for both thiophanate-methyl and carbendazim. These curves are then used to determine the concentration of the analytes in the fruit samples. For accurate results, the sample is first extracted with dichloromethane twice, then evaporated, and finally dissolved in a copper acetate solution. The mixture is heated under reflux before being extracted again with hydrochloric acid. The final step involves measuring the absorbance and comparing it to the standard curve to calculate the concentration in mg/kg. The formula used for calculation is: x = (V1 × C) / (m × V2) × 100 Where: - V1 = total volume of extraction solvent - C = standard concentration - m = sample weight - V2 = volume used in measurement Keywords: ultraviolet spectrophotometer, fruit, thiophanate-methyl, carbendazim, UV-1100, UV-1200 </dt> </div> <div class=

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